Human ERBB2 Knockdown Cell Line (Wb-Validated) - 产品中心

Human ERBB2 Knockdown Cell Line (Wb-Validated) #C64611

一键复制产品信息

返回列表

RT-qPCR analysis. HeLa cells were infected with ERBB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. ERBB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ERBB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

ERBB2
货号：

C64611
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Information

Picture

Citation(0)

Information

Product Name	Human ERBB2 Knockdown Cell Line (Wb-Validated)
Aliases	ERBB2; Erb-B2 Receptor Tyrosine Kinase 2; HER2; NEU; C-ERB-2; C-ERB2; MLN-19; HER-2; CD340; NGL; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Receptor Tyrosine-Protein Kinase ErbB-2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; P185(ErbB2); EC 2.7.10.1; MLN 19; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog); V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2; Neuroblastoma/Glioblastoma Derived Oncogene Homolog; Metastatic Lymph Node Gene 19; C-Erb B2/Neu Protein; CD340 Antigen; P185(ERBB2); HER-2/Neu; Herstatin; P185erbB2; EC 2.7.10; VSCN2; MLN19; TKR1
Background	Gene Name: ERBB2 NCBI Gene Entry: 2064
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human ERBB2 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with ERBB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ERBB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ERBB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

ERBB2
货号：

C64611
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页