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Human PDE1B Knockdown Cell Line (Wb-Validated) #C64847

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RT-qPCR analysis. HeLa cells were infected with PDE1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PDE1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PDE1B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PDE1B
  • 货号：
    C64847
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human PDE1B Knockdown Cell Line (Wb-Validated)
Aliases PDE1B; Phosphodiesterase 1B; PDES1B; Dual Specificity Calcium/Calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1B; Phosphodiesterase 1B, Calmodulin-Dependent; 63 KDa Cam-PDE; EC 3.1.4.17; Cam-PDE 1B; Presumed 63kDa Form Of The Type 1 Cyclic Nucleotide Phosphodiesterase Family Known As PDE1B; Calcium/Calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1B; Calcium/Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase; Epididymis Secretory Sperm Binding Protein Li 79p; Calmodulin-Stimulated Phosphodiesterase PDE1B1; HEL-S-79p; EC 3.1.4; PDE1B1
Background

Gene Name: PDE1B

NCBI Gene Entry: 5153
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PDE1B Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PDE1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PDE1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PDE1B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PDE1B
  • 货号：
    C64847
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PDE1B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PDE1B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PDE1B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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