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Human PSMA6 Knockdown Cell Line (Wb-Validated) #C64853

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RT-qPCR analysis. HeLa cells were infected with PSMA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSMA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PSMA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PSMA6
  • 货号：
    C64853
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human PSMA6 Knockdown Cell Line (Wb-Validated)
Aliases PSMA6; Proteasome 20S Subunit Alpha 6; PROS27; P27K; IOTA; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 6; Multicatalytic Endopeptidase Complex Iota Chain; Proteasome Subunit Alpha Type-6; Proteasome Subunit Alpha 6; 27 KDa Prosomal Protein; Proteasome Iota Chain; Macropain Iota Chain; MGC22756; MGC23846; MGC2333; PROS-27; Testicular Secretory Protein Li 44; Proteasome Subunit Alpha1; Proteasome Subunit Iota; Macropain Subunit Iota; Proteasome Subunit Α1; Prosomal P27K Protein; EC 3.4.25.1; Proteasome Subunit Alpha-1; Alpha-1
Background

Gene Name: PSMA6

NCBI Gene Entry: 5687
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PSMA6 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PSMA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PSMA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PSMA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PSMA6
  • 货号：
    C64853
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PSMA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSMA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PSMA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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