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Human NDUFB11 Knockdown Cell Line (Wb-Validated) #C64865

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RT-qPCR analysis. HeLa cells were infected with NDUFB11-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFB11 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFB11 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    NDUFB11
  • 货号：
    C64865
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human NDUFB11 Knockdown Cell Line (Wb-Validated)
Aliases NDUFB11; NADH:Ubiquinone Oxidoreductase Subunit B11; NP17.3; Np15; NADH Dehydrogenase [Ubiquinone] 1 Beta Subcomplex Subunit 11, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 11, 17.3kDa; NADH-Ubiquinone Oxidoreductase ESSS Subunit; Complex I NP17.3 Subunit; Neuronal Protein 17.3; Complex I-ESSS; CI-ESSS; P17.3; MC1DN30; Np17.3; ESSS
Background

Gene Name: NDUFB11

NCBI Gene Entry: 54539
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NDUFB11 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with NDUFB11-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. NDUFB11 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFB11 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    NDUFB11
  • 货号：
    C64865
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with NDUFB11-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NDUFB11 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NDUFB11 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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