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Human PIP4K2A Knockdown Cell Line (Wb-Validated) #C65034

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RT-qPCR analysis. HeLa cells were infected with PIP4K2A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. PIP4K2A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PIP4K2A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

PIP4K2A
货号：

C65034
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human PIP4K2A Knockdown Cell Line (Wb-Validated)
Aliases	PIP4K2A; Phosphatidylinositol-5-Phosphate 4-Kinase Type 2 Alpha; PIP5KIIalpha; PIP5KIIA; PIP5K2A; Phosphatidylinositol-4-Phosphate 5-Kinase, Type II, Alpha; Phosphatidylinositol 5-Phosphate 4-Kinase Type II Alpha; Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha; 1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Alpha; Diphosphoinositide Kinase 2-Alpha; PI(5)P 4-Kinase Type II Alpha; PIP4KII-Alpha; EC 2.7.1.149; PI5P4Kalpha; PIP5KIII; PI5P4KA; Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Alpha; 1-Phosphatidylinositol-4-Phosphate-5-Kinase; 1-Phosphatidylinositol-4-Phosphate Kinase; Phosphatidylinositol 5-Phosphate 4-Kinase; PtdIns(5)P-4-Kinase Isoform 2-Alpha; PtdIns(4)P-5-Kinase B Isoform; PtdIns(4)P-5-Kinase C Isoform; PIP5KII-Alpha; EC 2.7.1; PIP5K2; PIPK
Background	Gene Name: PIP4K2A NCBI Gene Entry: 5305
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human PIP4K2A Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with PIP4K2A-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PIP4K2A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PIP4K2A and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

PIP4K2A
货号：

C65034
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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