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Human CDK5RAP3 Knockdown Cell Line (Wb-Validated) #C65198

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RT-qPCR analysis. HT-1080 cells were infected with CDK5RAP3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CDK5RAP3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CDK5RAP3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    CDK5RAP3
  • 货号:
    C65198
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human CDK5RAP3 Knockdown Cell Line (Wb-Validated)
Aliases CDK5RAP3; CDK5 Regulatory Subunit Associated Protein 3; IC53; LZAP; OK/SW-Cl.114; MST016; HSF-27; C53; LXXLL/Leucine-Zipper-Containing ARF-Binding Protein; LXXLL/Leucine-Zipper-Containing ARFbinding Protein; Ischemic Heart CDK5 Activator-Binding Protein C53; CDK5 Regulatory Subunit-Associated Protein 3; FLJ13660; CDK5 Regulatory Subunit Associated Protein IC53-2; CDK5 Activator-Binding Protein C53; Protein HSF-27; PP1553
Background

Gene Name: CDK5RAP3

NCBI Gene Entry: 80279

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CDK5RAP3 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with CDK5RAP3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CDK5RAP3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CDK5RAP3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HT-1080 cells were infected with CDK5RAP3-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CDK5RAP3 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CDK5RAP3 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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