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Human CSNK1A1 Knockdown Cell Line (Wb-Validated) #C65200

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RT-qPCR analysis. HeLa cells were infected with CSNK1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CSNK1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CSNK1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    CSNK1A1
  • 货号：
    C65200
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human CSNK1A1 Knockdown Cell Line (Wb-Validated)
Aliases CSNK1A1; Casein Kinase 1 Alpha 1; CK1; CK1a; CKIa; Casein Kinase I Isoform Alpha; Clock Regulator Kinase; EC 2.7.11.1; CKI-Alpha; CK1alpha; CKIalpha; Epididymis Secretory Sperm Binding Protein Li 77p; Down-Regulated In Lung Cancer; Casein Kinase 1, Alpha; HEL-S-77p; HLCDGP1; PRO2975
Background

Gene Name: CSNK1A1

NCBI Gene Entry: 1452
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CSNK1A1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with CSNK1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. CSNK1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CSNK1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    CSNK1A1
  • 货号：
    C65200
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with CSNK1A1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. CSNK1A1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CSNK1A1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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