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Human CPSF6 Knockdown Cell Line (Wb-Validated) #C65204

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RT-qPCR analysis. HeLa cells were infected with CPSF6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.CPSF6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CPSF6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    CPSF6
  • 货号：
    C65204
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human CPSF6 Knockdown Cell Line (Wb-Validated)
Aliases CPSF6; Cleavage And Polyadenylation Specific Factor 6; CFIM68; Cleavage Factor Im Complex 68 KDa Subunit; HPBRII-4; HPBRII-7; CFIM72; CFIM; Cleavage And Polyadenylation Specificity Factor 68 KDa Subunit; Cleavage And Polyadenylation Specificity Factor Subunit 6; Cleavage And Polyadenylation Specific Factor 6, 68kDa; Pre-MRNA Cleavage Factor Im 68 KDa Subunit; CPSF 68 KDa Subunit; Protein HPBRII-4/7; Cleavage And Polyadenylation Specific Factor 6, 68kD Subunit; Pre-MRNA Cleavage Factor I, 68kD Subunit; Pre-MRNA Cleavage Factor Im (68kD); CFIm68
Background

Gene Name: CPSF6

NCBI Gene Entry: 11052
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human CPSF6 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with CPSF6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.CPSF6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CPSF6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    CPSF6
  • 货号：
    C65204
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with CPSF6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.CPSF6 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against CPSF6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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