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Human EFEMP1 Knockdown Cell Line (Wb-Validated) #C65222

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RT-qPCR analysis. HeLa cells were infected with EFEMP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.EFEMP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EFEMP1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
  • 基因名:
    EFEMP1
  • 货号:
    C65222
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human EFEMP1 Knockdown Cell Line (Wb-Validated)
Aliases EFEMP1; EGF Containing Fibulin Extracellular Matrix Protein 1; FBLN3; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; S1-5; MTLV; FBNL; EGF Containing Fibulin Like Extracellular Matrix Protein 1; Extracellular Protein S1-5; Fibulin-3; FIBL-3; DHRD; Glaucoma 1, Open Angle, H (Adult-Onset); Fibrillin-Like Protein; Fibrillin-Like; Fibulin 3; ARCL1D; GLC1H; DRAD; MLVT
Background

Gene Name: EFEMP1

NCBI Gene Entry: 2202

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human EFEMP1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with EFEMP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.EFEMP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EFEMP1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with EFEMP1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.EFEMP1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EFEMP1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
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