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Human PIP5K1C Knockdown Cell Line (Wb-Validated) #C65435

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RT-qPCR analysis. HeLa cells were infected with PIP5K1C-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.PIP5K1C protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PIP5K1C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PIP5K1C
  • 货号：
    C65435
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human PIP5K1C Knockdown Cell Line (Wb-Validated)
Aliases PIP5K1C; Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Gamma; PIP5Kgamma; KIAA0589; LCCS3; Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, Gamma; Phosphatidylinositol 4-Phosphate 5-Kinase Type-1 Gamma; Type I Phosphatidylinositol 4-Phosphate 5-Kinase Gamma; PtdIns(4)P-5-Kinase 1 Gamma; PIP5K1gamma; EC 2.7.1.68; Diphosphoinositide Kinase; Type I PIP Kinase; PIP5K1-Gamma; PIP5K-GAMMA; EC 2.7.1
Background

Gene Name: PIP5K1C

NCBI Gene Entry: 23396
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PIP5K1C Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PIP5K1C-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.PIP5K1C protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PIP5K1C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PIP5K1C
  • 货号：
    C65435
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PIP5K1C-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.PIP5K1C protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PIP5K1C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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