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Human YTHDF1 Knockdown Cell Line (Wb-Validated) #C65668

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RT-qPCR analysis. HeLa cells were infected with YTHDF1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.YTHDF1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against THDF1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
  • 基因名：
    YTHDF1
  • 货号：
    C65668
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human YTHDF1 Knockdown Cell Line (Wb-Validated)
Aliases YTHDF1; YTH N6-Methyladenosine RNA Binding Protein F1; C20orf21; Dermatomyositis Associated With Cancer Putative Autoantigen 1; YTH N(6)-Methyladenosine RNA Binding Protein 1; YTH N6-Methyladenosine RNA Binding Protein 1; YTH Domain-Containing Family Protein 1; YTH Domain Family, Member 1; YTH Domain Family 1; FLJ20391; DACA-1; DF1; YTH Domain Family Protein 1
Background

Gene Name: YTHDF1

NCBI Gene Entry: 54915
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human YTHDF1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with YTHDF1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.YTHDF1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against THDF1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    YTHDF1
  • 货号：
    C65668
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with YTHDF1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.YTHDF1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against THDF1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
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