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Human EHMT2 Knockdown Cell Line (Wb-Validated) #C68352

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RT-qPCR analysis. MCF7 cells were infected with EHMT2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. EHMT2 protein expression in wild-type (WT) and shRNA knockdown (KD) MCF7 cells was detected using Western blotting. TUBB served as a loading control. The blots were incubated with primary antibodies against EHMT2 and TUBB, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    EHMT2
  • 货号：
    C68352
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human EHMT2 Knockdown Cell Line (Wb-Validated)
Aliases EHMT2; Euchromatic Histone Lysine Methyltransferase 2; KMT1C; G9A; C6orf30; BAT8; Euchromatic Histone-Lysine N-Methyltransferase 2; Histone-Lysine N-Methyltransferase EHMT2; Histone H3-K9 Methyltransferase 3; HLA-B Associated Transcript 8; Lysine N-Methyltransferase 1C; H3-K9-HMTase 3; Em:AF134726.3; NG36/G9a; NG36; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3; Chromosome 6 Open Reading Frame 30; Ankyrin Repeat-Containing Protein; G9A Histone Methyltransferase; HLA-B-Associated Transcript 8; EC 2.1.1.367; Protein G9a; EC 2.1.1.-; GAT8
Background

Gene Name: EHMT2

NCBI Gene Entry: 10919
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human EHMT2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. MCF7 cells were infected with EHMT2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. EHMT2 protein expression in wild-type (WT) and shRNA knockdown (KD) MCF7 cells was detected using Western blotting. TUBB served as a loading control. The blots were incubated with primary antibodies against EHMT2 and TUBB, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    EHMT2
  • 货号：
    C68352
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. MCF7 cells were infected with EHMT2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. EHMT2 protein expression in wild-type (WT) and shRNA knockdown (KD) MCF7 cells was detected using Western blotting. TUBB served as a loading control. The blots were incubated with primary antibodies against EHMT2 and TUBB, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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