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Human NFKB1 Knockdown Cell Line (Wb-Validated) #C85241

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RT-qPCR analysis. HeLa cells were infected with NFKB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NFKB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#61524, 1:5,000) against NFKB1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    NFKB1
  • 货号：
    C85241
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human NFKB1 Knockdown Cell Line (Wb-Validated)
Aliases NFKB1; Nuclear Factor Kappa B Subunit 1; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear Factor NF-Kappa-B P105 Subunit; NFKB-P50; NF-KB1; KBF1; DNA-Binding Factor KBF1; NF-KappaB; NFkappaB; EBP-1; P105; P50; Nuclear Factor Kappa-B DNA Binding Subunit; Nuclear Factor NF-Kappa-B P50 Subunit; NF-Kappabeta; NF-Kappa-B1; NFKB-P105; NF-KAPPAB; NFKAPPAB; CVID12; NF-KB
Background

Gene Name: NFKB1

NCBI Gene Entry: 4790
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NFKB1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with NFKB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. NFKB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#61524, 1:5,000) against NFKB1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    NFKB1
  • 货号：
    C85241
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with NFKB1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. NFKB1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#61524, 1:5,000) against NFKB1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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