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Anti-HA Tag Mouse Monoclonal Antibody #T013

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Immunocytochemical analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of over-expressed HA-tagged protein in 293T cell lysate.
  • 基因名：
  • 货号：
    T013
  • 应用：
    WB
  • 物种反应性：
    Recombinant protein
规格：
20 μL，50 μL，100 μL
价格：
¥ 310.00，¥ 600.00，¥ 1,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-HA Tag Mouse Monoclonal Antibody
Aliases HA-tag; HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2
Background The HA-tag is a small, widely used epitope tag derived from the hemagglutinin (HA) glycoprotein of the human influenza virus. The most common HA-tag sequence (YPYDVPDYA, 9 amino acids) corresponds to an immunodominant epitope recognized by monoclonal antibodies. It is typically fused to the N-terminus or C-terminus of recombinant proteins via genetic engineering. Unlike larger fusion tags (e.g., GFP), its compact size minimizes interference with protein structure or function while enabling highly specific detection.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Mouse monoclonal antibody

Species Reactivity: Recombinant protein

Applications Tested: Western Blotting (WB), immunocytochemistry (IC), immunoprecipitation (IP)
Immunogen A synthesized peptide derived from HA-tag
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:5,000
Immunocytochemistry (IC): 1:200-1:500
Immunoprecipitation (IP): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of over-expressed HA-tagged protein in 293T cell lysate.
Citation(0)
  • 基因名：
  • 货号：
    T013
  • 应用：
    WB
  • 物种反应性：
    Recombinant protein
规格：
20 μL，50 μL，100 μL
价格：
¥ 310.00，¥ 600.00，¥ 1,000.00
RELATED PRODUCTS
Immunocytochemical analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of over-expressed HA-tagged protein in 293T cell lysate.
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