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Anti-V5 Tag Mouse Monoclonal Antibody #T017

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Immunocytochemical analysis of 293 cells transfected with a V5-tag protein(1:2,000). Blue: DAPI, red: anti-V5.
1μg V5 fusion protein+Primary antibody dilution at 1 (1:5,000), 2 (1:10,000).
  • 基因名:
  • 货号:
    T017
  • 应用:
    WB
  • 物种反应性:
    Recombinant protein
规格:
20 μL,50 μL,100 μL
价格:
¥ 310.00,¥ 600.00,¥ 1,000.00
Information
Product Name Anti-V5 Tag Mouse Monoclonal Antibody
Aliases V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag
Background The V5-tag is a short, artificial peptide sequence (GKPIPNPLLGLDST) derived from a small epitope present in the P and V proteins of the paramyxovirus simian virus 5 (SV5). It is genetically engineered and fused to either the N-terminus or C-terminus of a recombinant protein of interest, primarily serving as an epitope tag. Unlike affinity tags like the His-tag, its main purpose is not purification but rather highly specific detection and localization.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Mouse monoclonal antibody

Species Reactivity: Recombinant protein

Applications Tested: Western Blotting (WB), immunocytochemistry (IC), immunoprecipitation (IP)

Immunogen A synthesized peptide derived from V5-Tag
Storage Buffer Supplied in PBS (pH7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:5,000
Immunocytochemistry (IC): 1:200-1:1,000
Immunoprecipitation (IP): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of 293 cells transfected with a V5-tag protein(1:2,000). Blue: DAPI, red: anti-V5.
  • 1μg V5 fusion protein+Primary antibody dilution at 1 (1:5,000), 2 (1:10,000).
Citation(0)
Immunocytochemical analysis of 293 cells transfected with a V5-tag protein(1:2,000). Blue: DAPI, red: anti-V5.
1μg V5 fusion protein+Primary antibody dilution at 1 (1:5,000), 2 (1:10,000).
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