Western blotting analysis using anti-GST tag antibody (Cat#T027). GST-tagged fusion protein (0.50 μg) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-GST tag antibody (Cat#T027, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).