| Product Name |
Anti-GFP Tag Mouse Monoclonal Antibody |
| Aliases |
GFP; yfp; Green fluorescent protein |
| Background |
The GFP (Green Fluorescent Protein) tag is a 27 kDa fluorescent protein derived from the jellyfish Aequorea victoria. It emits bright green light (λ~509 nm) upon exposure to blue or UV light, enabling real-time visualization of tagged proteins in live or fixed cells without additional substrates. Widely used as a reporter gene, GFP-tagging allows non-invasive tracking of protein localization, expression dynamics, and interactions across diverse organisms (e.g., bacteria, mammalian cells). Its engineered variants (e.g., EGFP, mCherry) offer enhanced brightness, photostability, and spectral versatility for multiplex imaging. |
| Application Information |
Molecular Weight: Predicted, 27
Clonality: Mouse monoclonal antibody
Species Reactivity: Recombinant protein
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
|
| Immunogen |
Recombinant protein of GFP |
| Isotype |
Mouse IgG1 |
| Storage Buffer |
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide. |
| Storage |
Store at -20 °C for one year. |
| Recommended Dilutions |
Western Blotting (WB): 1:1,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000 |
| Note |
This product is for research use only. |
