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Anti-Phospho-ZAP70 (Y315) Rabbit Polyclonal Antibody #U0315

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Immunohistochemical analysis of ZAP70 (Phospho-Y315) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of ZAP70 (Phospho-Y315) expression in Jurkat H2O2-treated (A), HeLa H2O2-treated (B), Ramos lambda phosphatase-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates.
  • 基因名:
    ZAP70
  • 货号:
    U0315
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, C, Mk, P, Rb
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Phospho-ZAP70 (Y315) Rabbit Polyclonal Antibody
Aliases SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
Background

Gene Name: ZAP70

NCBI Gene Entry: 7535

UniProt Entry: P43403

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 70 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, monkey, pig, rabbit

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Phospho-ZAP70 (Y315)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of ZAP70 (Phospho-Y315) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of ZAP70 (Phospho-Y315) expression in Jurkat H2O2-treated (A), HeLa H2O2-treated (B), Ramos lambda phosphatase-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates.
Citation(0)
Immunohistochemical analysis of ZAP70 (Phospho-Y315) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of ZAP70 (Phospho-Y315) expression in Jurkat H2O2-treated (A), HeLa H2O2-treated (B), Ramos lambda phosphatase-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates.
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