Immunohistochemical analysis of 4EBP1 (Phospho-T46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of 4EBP1 (Phospho-T46) expression in K562 PMA-treated (A), NIH3T3 PMA-treated (B), PC12 PMA-treated (C) whole cell lysates.
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Species Reactivity: Human, mouse, rat, bovine, pig, zebrafish
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human Phospho-4EBP1 (T46)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunohistochemistry (IHC): 1:100-1:200
Note
This product is for research use only.
Picture
Immunohistochemical analysis of 4EBP1 (Phospho-T46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of 4EBP1 (Phospho-T46) expression in K562 PMA-treated (A), NIH3T3 PMA-treated (B), PC12 PMA-treated (C) whole cell lysates.
Immunohistochemical analysis of 4EBP1 (Phospho-T46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of 4EBP1 (Phospho-T46) expression in K562 PMA-treated (A), NIH3T3 PMA-treated (B), PC12 PMA-treated (C) whole cell lysates.
