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Anti-Phospho-NF-kappaB p105 (S932) Rabbit Polyclonal Antibody #U0397

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Immunohistochemical analysis of NF-kappaB p105 (Phospho-S932) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of NF-kappaB p105 (Phospho-S932) expression in HeLa LPS-treated (A), NIH3T3 LPS-treated (B), Raw264.7 LPS-treated (C), rat kidney (D) whole cell lysates.
  • 基因名:
    NFKB1
  • 货号:
    U0397
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, C, D, P
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Phospho-NF-kappaB p105 (S932) Rabbit Polyclonal Antibody
Aliases Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Background

Gene Name: NFKB1

NCBI Gene Entry: 4790

UniProt Entry: P19838

Application Information

Molecular Weight: Predicted, 105 kDa; observed, 105 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, dog, pig

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Phospho-NF-kappaB p105 (S932)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of NF-kappaB p105 (Phospho-S932) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of NF-kappaB p105 (Phospho-S932) expression in HeLa LPS-treated (A), NIH3T3 LPS-treated (B), Raw264.7 LPS-treated (C), rat kidney (D) whole cell lysates.
Citation(0)
Immunohistochemical analysis of NF-kappaB p105 (Phospho-S932) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of NF-kappaB p105 (Phospho-S932) expression in HeLa LPS-treated (A), NIH3T3 LPS-treated (B), Raw264.7 LPS-treated (C), rat kidney (D) whole cell lysates.
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