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Anti-Phospho-RPS6 (S235) Rabbit Polyclonal Antibody #U0410

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Immunocytochemical analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.
  • 基因名：
    RPS6
  • 货号：
    U0410
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, Mk, S, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-RPS6 (S235) Rabbit Polyclonal Antibody
Aliases 40S ribosomal protein S6; Phosphoprotein NP33
Background

Gene Name: RPS6

NCBI Gene Entry: 6194

UniProt Entry: P62753
Application Information

Molecular Weight: Predicted, 28 kDa; observed, 32 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, monkey, sheep, zebrafish

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-RPS6 (S235)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:100-1:500
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.
Citation(0)
  • 基因名：
    RPS6
  • 货号：
    U0410
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, Mk, S, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.
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