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Anti-Phospho-Histone H3 (T11) Rabbit Polyclonal Antibody #U0437

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Immunocytochemical analysis of Histone H3 (Phospho-T11) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blotting analysis of Histone H3 (Phospho-T11) expression in HepG2 EGF-treated (A), SP2/0 EGF-treated (B), rat brain (C) whole cell lysates.
  • 基因名：
    HIST1H3A
  • 货号：
    U0437
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, P, Rb, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-Histone H3 (T11) Rabbit Polyclonal Antibody
Aliases HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Background

Gene Name: HIST1H3A

NCBI Gene Entry: 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968

UniProt Entry: P68431/Q71DI3/P84243
Application Information

Molecular Weight: Predicted, 15 kDa; observed, 17 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, pig, rabbit, zebrafish

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-Histone H3 (T11)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:100-1:500
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of Histone H3 (Phospho-T11) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
  • Western blotting analysis of Histone H3 (Phospho-T11) expression in HepG2 EGF-treated (A), SP2/0 EGF-treated (B), rat brain (C) whole cell lysates.
Citation(0)
  • 基因名：
    HIST1H3A
  • 货号：
    U0437
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, P, Rb, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of Histone H3 (Phospho-T11) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blotting analysis of Histone H3 (Phospho-T11) expression in HepG2 EGF-treated (A), SP2/0 EGF-treated (B), rat brain (C) whole cell lysates.
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