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Anti-Phospho-Histone Deacetylase 5/9 (S259/220) Rabbit Polyclonal Antibody #U0559

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Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates.
  • 基因名:
    HDAC5/9
  • 货号:
    U0559
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, B, C, D, P, Rb
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Phospho-Histone Deacetylase 5/9 (S259/220) Rabbit Polyclonal Antibody
Aliases HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR
Background

Gene Name: HDAC5/9

NCBI Gene Entry: 10014/9734

UniProt Entry: Q9UQL6/Q9UKV0

Application Information

Molecular Weight: Predicted, 121, 111 kDa; observed, 124 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, bovine, chicken, dog, pig, rabbit

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Phospho-Histone Deacetylase 5/9 (S259/220)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates.
Citation(0)
Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates.
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