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Anti-Phospho-SMAD2 (T220) Rabbit Polyclonal Antibody #U0857

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Immunohistochemical analysis of SMAD2 (Phospho-T220) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of SMAD2 (Phospho-T220) expression in rat heart (A) whole cell lysates.
  • 基因名：
    SMAD2
  • 货号：
    U0857
  • 应用：
    WB, IHC, IC
  • 物种反应性：
    H, M, R, B, C, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-SMAD2 (T220) Rabbit Polyclonal Antibody
Aliases MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Background

Gene Name: SMAD2

NCBI Gene Entry: 4087

UniProt Entry: Q15796
Application Information

Molecular Weight: Predicted, 52 kDa; observed, 60 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, zebrafish

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-SMAD2 (T220)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:200
Immunocytochemistry (IC): 1:100-1:500
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of SMAD2 (Phospho-T220) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of SMAD2 (Phospho-T220) expression in rat heart (A) whole cell lysates.
Citation(0)
  • 基因名：
    SMAD2
  • 货号：
    U0857
  • 应用：
    WB, IHC, IC
  • 物种反应性：
    H, M, R, B, C, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunohistochemical analysis of SMAD2 (Phospho-T220) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of SMAD2 (Phospho-T220) expression in rat heart (A) whole cell lysates.
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