Immunohistochemical analysis of SMAD2 (Phospho-S255) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of SMAD2 (Phospho-S255) expression in K562 (A) whole cell lysates.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP)
Immunogen
A synthesized peptide derived from human Phospho-SMAD2 (S255)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunohistochemistry (IHC): 1:50-1:100 Immunoprecipitation (IP): 1:10-1:50
Note
This product is for research use only.
Picture
Immunohistochemical analysis of SMAD2 (Phospho-S255) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of SMAD2 (Phospho-S255) expression in K562 (A) whole cell lysates.
Immunohistochemical analysis of SMAD2 (Phospho-S255) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of SMAD2 (Phospho-S255) expression in K562 (A) whole cell lysates.
