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Anti-Phospho-NF-κB p65 (S536) Rabbit Polyclonal Antibody #U2271

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Western blotting analysis of lysates from 1) A549, 2) 3T3, 3) 293T , 4)K562 cells, (Green) primary antibody was diluted at 1:1,000, 4℃ over night, secondary antibody was diluted at 1:10,000, 37℃ 1 hour. (Red) Actin β antibody was diluted at 1:5,000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10,000, 37℃ 1 hour.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 基因名：
    RELA
  • 货号：
    U2271
  • 应用：
    WB, IHC, IC, IP
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-NF-κB p65 (S536) Rabbit Polyclonal Antibody
Aliases RELA; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; NFKB3; P65; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; Transcription Factor P65; NF-Kappa-B Transcription Factor P65; NF-Kappa-B P65delta3; AIF3BL3; CMCU
Background

Gene Name: RELA

NCBI Gene Entry: 5970

UniProt Entry: Q04206
Application Information

Molecular Weight: Predicted, 60 kDa; observed, 60 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC), immunoprecipitation (IP)
Immunogen A synthesized peptide derived from human Phospho-NF-κB p65 (S536)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:300
Immunocytochemistry (IC): 1:50-1:200
Immunoprecipitation (IP): 2-5 μg/mg
Note This product is for research use only.
Picture
  • Western blotting analysis of lysates from 1) A549, 2) 3T3, 3) 293T , 4)K562 cells, (Green) primary antibody was diluted at 1:1,000, 4℃ over night, secondary antibody was diluted at 1:10,000, 37℃ 1 hour. (Red) Actin β antibody was diluted at 1:5,000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10,000, 37℃ 1 hour.
  • Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Citation(0)
  • 基因名：
    RELA
  • 货号：
    U2271
  • 应用：
    WB, IHC, IC, IP
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Western blotting analysis of lysates from 1) A549, 2) 3T3, 3) 293T , 4)K562 cells, (Green) primary antibody was diluted at 1:1,000, 4℃ over night, secondary antibody was diluted at 1:10,000, 37℃ 1 hour. (Red) Actin β antibody was diluted at 1:5,000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10,000, 37℃ 1 hour.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200 (4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. Phospho-NFκB-p65 (S536) antibody was diluted at 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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