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Anti-Bak Rabbit Monoclonal Antibody #1113

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Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Green, isotype control; red, Bak.
Immunocytochemical staining of HepG2 cells with Bak antibody (Cat#1113, 1:1,000). Nuclei were stained blue with DAPI; Bak was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    BAK1
  • 货号：
    1113
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information
Product Name Anti-Bak Rabbit Monoclonal Antibody
Aliases BAK1; BCL2 Antagonist/Killer 1; BCL2L7; BAK; CDN1; Bcl-2 Homologous Antagonist/Killer; Apoptosis Regulator BAK; Bcl-2-Like Protein 7; Bcl2-L-7; Pro-Apoptotic Protein BAK; BCL2-Antagonist/Killer 1; BCL2-Like 7 Protein; BAK-LIKE
Background

Gene Name: BAK1

NCBI Gene Entry: 578

UniProt Entry: Q16611
Application Information

Molecular Weight: Predicted, 23 kDa; observed, 23 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB7960

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Bak
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Green, isotype control; red, Bak.
  • Immunocytochemical staining of HepG2 cells with Bak antibody (Cat#1113, 1:1,000). Nuclei were stained blue with DAPI; Bak was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    BAK1
  • 货号：
    1113
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Bak expression in HepG2 cells using Bak antibody (Cat#1113, 1:2,000). Green, isotype control; red, Bak.
Immunocytochemical staining of HepG2 cells with Bak antibody (Cat#1113, 1:1,000). Nuclei were stained blue with DAPI; Bak was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Bak antibody (Cat#1113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bak antibody (Cat#1113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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