Anti-BCL2 binding component 3 Rabbit Monoclonal Antibody - 产品中心

Anti-BCL2 binding component 3 Rabbit Monoclonal Antibody #1119

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Flow cytometric analysis of BCL2 binding component 3 expression in HepG2 cells using BCL2 binding component 3 antibody (Cat#1119, 1:2,000). Green, isotype control; red, BCL2 binding component 3.

Immunocytochemical staining of HepG2 cells with BCL2 binding component 3 antibody (Cat#1119, 1:1,000). Nuclei were stained blue with DAPI; BCL2 binding component 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-BCL2 binding component 3 antibody (Cat#1119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL2 binding component 3 antibody (Cat#1119, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BBC3
货号：

1119
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-BCL2 binding component 3 Rabbit Monoclonal Antibody
Aliases	BBC3; BCL2 Binding Component 3; PUMA; JFY1; P53 Up-Regulated Modulator Of Apoptosis; P53-Upregulated Modulator Of Apoptosis; JFY-1; Bcl-2-Binding Component 3, Isoforms 3/4; Bcl-2-Binding Component 3, Isoforms 1/2; Bcl-2-Binding Component 3
Background	Gene Name: BBC3 NCBI Gene Entry: 27113 UniProt Entry: Q9BXH1
Application Information	Molecular Weight: Predicted, 26 kDa; observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1935 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PUMA
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BCL2 binding component 3 expression in HepG2 cells using BCL2 binding component 3 antibody (Cat#1119, 1:2,000). Green, isotype control; red, BCL2 binding component 3.
Immunocytochemical staining of HepG2 cells with BCL2 binding component 3 antibody (Cat#1119, 1:1,000). Nuclei were stained blue with DAPI; BCL2 binding component 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-BCL2 binding component 3 antibody (Cat#1119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL2 binding component 3 antibody (Cat#1119, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BBC3
货号：

1119
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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