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Anti-CYP24A1 Rabbit Monoclonal Antibody #1448

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Flow cytometric analysis of CYP24A1 expression in H9c2 cells using CYP24A1 antibody (Cat#1448, 1:2,000). Green, isotype control; red, CYP24A1.
Immunocytochemical staining of H9c2 cells with CYP24A1 antibody (Cat#1448, 1:1,000). Nuclei were stained blue with DAPI; CYP24A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CYP24A1 antibody (Cat#1448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP24A1 antibody (Cat#1448, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CYP24A1
  • 货号:
    1448
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CYP24A1 Rabbit Monoclonal Antibody
Aliases CYP24A1; Cytochrome P450 Family 24 Subfamily A Member 1; P450-CC24 ; CYP24; CP24; Cytochrome P450, Subfamily XXIV (Vitamin D 24-Hydroxylase); 1,25-Dihydroxyvitamin D(3) 24-Hydroxylase, Mitochondrial; Cytochrome P450, Family 24, Subfamily A, Polypeptide 1; Vitamin D(3) 24-Hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24; LncBCAS1-4_1; 24-OHase; 1,25-@Dihydroxyvitamin D3 24-Hydroxylase; Exo-Mitochondrial Protein; Vitamin D 24-Hydroxylase; EC 1.14.15.16; LNCBCAS1-4_1; HCINF1; HCAI
Background

Gene Name: CYP24A1

NCBI Gene Entry: 1591

UniProt Entry: Q07973

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3795

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CYP24A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CYP24A1 expression in H9c2 cells using CYP24A1 antibody (Cat#1448, 1:2,000). Green, isotype control; red, CYP24A1.
  • Immunocytochemical staining of H9c2 cells with CYP24A1 antibody (Cat#1448, 1:1,000). Nuclei were stained blue with DAPI; CYP24A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-CYP24A1 antibody (Cat#1448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP24A1 antibody (Cat#1448, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CYP24A1 expression in H9c2 cells using CYP24A1 antibody (Cat#1448, 1:2,000). Green, isotype control; red, CYP24A1.
Immunocytochemical staining of H9c2 cells with CYP24A1 antibody (Cat#1448, 1:1,000). Nuclei were stained blue with DAPI; CYP24A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CYP24A1 antibody (Cat#1448). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP24A1 antibody (Cat#1448, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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