Anti-14-3-3 alpha/beta Rabbit Monoclonal Antibody - 产品中心

Anti-14-3-3 alpha/beta Rabbit Monoclonal Antibody #1128

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Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#1128, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.

Immunocytochemical staining of HepG2 cells with 14-3-3 alpha/beta antibody (Cat#1128, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#1128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

YWHAB
货号：

1128
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-14-3-3 alpha/beta Rabbit Monoclonal Antibody
Aliases	YWHAB; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Protein Beta/Alpha; 14-3-3 Alpha; Protein 1054; KCIP-1; YWHAA; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Epididymis Secretory Protein Li 1; 14-3-3 Beta; HEL-S-1; GW128; HS1
Background	Gene Name: YWHAB NCBI Gene Entry: 7529 UniProt Entry: P31946
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1975 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human 14-3-3 alpha + beta
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (Cat#1128, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.
Immunocytochemical staining of HepG2 cells with 14-3-3 alpha/beta antibody (Cat#1128, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-14-3-3 alpha/beta antibody (Cat#1128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 alpha/beta antibody (Cat#1128, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

YWHAB
货号：

1128
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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