Flow cytometric analysis of Caspase 3 p12 expression in C2C12 cells using anti-Caspase 3 p12 antibody (Cat#4211, 1:2,000). Green, isotype control; red, Caspase 3 p12.
Immunocytochemical staining of C2C12 cells with anti-Caspase 3 p12 antibody (Cat#4211, 1:1,000) . Nuclei were stained blue with DAPI; Caspase 3 p12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-caspase 3 p12 antibody (Cat#4211). Total cell lysates (30 μg) from various cell lines (In the right figure, Lane 1: untreated A549, Lane 2: A549 treated with TAPP for 18 hours followed by irradiation with 660 nm laser for 5 minutes)were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 p12 antibody (Cat#4211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 p12 antibody (Cat#4211, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
