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Anti-MAP3K5 Rabbit Monoclonal Antibody #1160

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Flow cytometric analysis of MAP3K5 expression in HT-1080 cells using MAP3K5 antibody (Cat#1160, 1:2,000). Green, isotype control; red, MAP3K5.
Immunocytochemical staining of HT-1080 cells with MAP3K5 antibody (Cat#1160, 1:1,000). Nuclei were stained blue with DAPI; MAP3K5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MAP3K5 antibody (Cat#1160). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K5 antibody (Cat#1160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). MAP3K5, mitogen-activated protein kinase kinase kinase 5.
  • 基因名:
    MAP3K5
  • 货号:
    1160
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MAP3K5 Rabbit Monoclonal Antibody
Aliases MAP3K5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPKKK5; ASK1; MEKK5; Apoptosis Signal-Regulating Kinase 1; MAPK/ERK Kinase Kinase 5; MEK Kinase 5; EC 2.7.11.25; MEKK 5; ASK-1; Apoptosis Signal Regulating Kinase 1; MAP/ERK Kinase Kinase 5; EC 2.7.11
Background

Gene Name: MAP3K5

NCBI Gene Entry: 4217

UniProt Entry: Q99683

Application Information

Molecular Weight: Predicted, 155 kDa; observed, 155 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2060

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ASK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MAP3K5 expression in HT-1080 cells using MAP3K5 antibody (Cat#1160, 1:2,000). Green, isotype control; red, MAP3K5.
  • Immunocytochemical staining of HT-1080 cells with MAP3K5 antibody (Cat#1160, 1:1,000). Nuclei were stained blue with DAPI; MAP3K5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-MAP3K5 antibody (Cat#1160). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K5 antibody (Cat#1160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). MAP3K5, mitogen-activated protein kinase kinase kinase 5.
Citation(0)
Flow cytometric analysis of MAP3K5 expression in HT-1080 cells using MAP3K5 antibody (Cat#1160, 1:2,000). Green, isotype control; red, MAP3K5.
Immunocytochemical staining of HT-1080 cells with MAP3K5 antibody (Cat#1160, 1:1,000). Nuclei were stained blue with DAPI; MAP3K5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MAP3K5 antibody (Cat#1160). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K5 antibody (Cat#1160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). MAP3K5, mitogen-activated protein kinase kinase kinase 5.
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