Western blotting analysis using anti-Ki-67 antibody (Cat#52514). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ki-67 antibody (Cat#52514, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 antibody, Unconjugated at 1:200 overnight at 4°C.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Ki-67
Isotype
Rabbit IgG
Storage Buffer
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% proclin300 and 50% glycerol.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry (IHC): 1:200-1:800
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Ki-67 antibody (Cat#52514). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ki-67 antibody (Cat#52514, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 antibody, Unconjugated at 1:200 overnight at 4°C.
Western blotting analysis using anti-Ki-67 antibody (Cat#52514). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ki-67 antibody (Cat#52514, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 antibody, Unconjugated at 1:200 overnight at 4°C.
