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Anti-FosB Rabbit Monoclonal Antibody #1225

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Flow cytometric analysis of FosB expression in HepG2 cells using anti-FosB antibody (Cat#1225, 1:2,000). Green, isotype control; red, FosB.

Immunocytochemical staining of HepG2 cells with anti-FosB antibody (Cat#1225, 1:1,000). Nuclei were stained blue with DAPI; FosB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-FosB antibody (Cat#1225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FosB antibody (Cat#1225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FOSB
货号：

1225
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-FosB Rabbit Monoclonal Antibody
Aliases	FOSB; FosB Proto-Oncogene, AP-1 Transcription Factor Subunit; G0S3; GOSB; GOS3; AP-1; FBJ Murine Osteosarcoma Viral Oncogene Homolog B; Transcription Factor AP-1 Subunit FosB; G0/G1 Switch Regulatory Protein 3; Activator Protein 1; Protein FosB; MGC42291; FosB Proto-Oncogene, AP-1 Trancription Factor Subunit; Oncogene FOS-B; DKFZp686C0818; DKFZP686C0818
Background	Gene Name: FOSB NCBI Gene Entry: 2354 UniProt Entry: P53539
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 48 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2285 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Fos B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM) : 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FosB expression in HepG2 cells using anti-FosB antibody (Cat#1225, 1:2,000). Green, isotype control; red, FosB.
Immunocytochemical staining of HepG2 cells with anti-FosB antibody (Cat#1225, 1:1,000). Nuclei were stained blue with DAPI; FosB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-FosB antibody (Cat#1225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FosB antibody (Cat#1225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FOSB
货号：

1225
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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