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Anti-Keratin 7 Rabbit Monoclonal Antibody #1270

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Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#1270, 1:2,000). Green, isotype control; red, Keratin 7.
Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#1270, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Keratin 7 antibody (Cat#1270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 7 antibody (Cat#1270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#1270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    KRT7
  • 货号：
    1270
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Keratin 7 Rabbit Monoclonal Antibody
Aliases Keratin 7; CK-7; SCL; K7; Keratin, Type II Cytoskeletal 7; Sarcolectin 2; K2C7 2; CK7 2; Keratin, 55K Type II; Cytoskeletal; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; Keratin, Simple Epithelial Type I, K7; Type II Mesothelial Keratin K7; Cytokeratin-7; Keratin-7
Background

Gene Name: KRT7

NCBI Gene Entry: 3855

UniProt Entry: P08729
Application Information

Molecular Weight: Predicted, 51 kDa; observed, 51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2930

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Cytokeratin 7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#1270, 1:2,000). Green, isotype control; red, Keratin 7.
  • Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#1270, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Keratin 7 antibody (Cat#1270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 7 antibody (Cat#1270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#1270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    KRT7
  • 货号：
    1270
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Keratin 7 expression in HepG2 cells using Keratin 7 antibody (Cat#1270, 1:2,000). Green, isotype control; red, Keratin 7.
Immunocytochemical staining of HepG2 cells with Keratin 7 antibody (Cat#1270, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Keratin 7 antibody (Cat#1270). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 7 antibody (Cat#1270, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-glial fibrillary acidic protein antibody (Cat#1270, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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