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Anti-NALP3 Rabbit Monoclonal Antibody #1274

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Flow cytometric analysis of NALP3 expression in HT-1080 cells using NALP3 antibody (Cat#1274, 1:2,000). Green, isotype control; red, NALP3.

Immunocytochemical staining of HT-1080 cells with anti-NALP3 antibody (Cat#1274, 1:1,000) . Nuclei were stained blue with DAPI; NALP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-NALP3 antibody (Cat#1274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NALP3 antibody (Cat#1274, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NLRP3
货号：

1274
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-NALP3 Rabbit Monoclonal Antibody
Aliases	NLRP3; NLR Family Pyrin Domain Containing 3; PYPAF1; CLR1.1; NALP3; Cryopyrin; AGTAVPRL; CIAS1; FCAS; AII; AVP; FCU; MWS; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; Cold-Induced Autoinflammatory Syndrome 1 Protein; NACHT, LRR And PYD Domains-Containing Protein 3; PYRIN-Containing APAF1-Like Protein 1; Deafness, Autosomal Dominant 34; Caterpiller Protein 1.1; C1orf7; DFNA34; NACHT Domain-, Leucine-Rich Repeat-, And PYD-Containing Protein 3; Cryopyrin, NACHT, LRR And PYD Domains - Containing Protein 3; Angiotensin/Vasopressin Receptor AII/AVP-Like; Cold Autoinflammatory Syndrome 1 Protein; NACHT, LRR And PYD Containing Protein 3; Cold Autoinflammatory Syndrome 1; EC 3.6.4.-; C1ORF7; FCAS1; KEFH
Background	Gene Name: NLRP3 NCBI Gene Entry: 114548 UniProt Entry: Q96P20
Application Information	Molecular Weight: Predicted, 118 kDa; observed, 118 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2540 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NALP3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NALP3 expression in HT-1080 cells using NALP3 antibody (Cat#1274, 1:2,000). Green, isotype control; red, NALP3.
Immunocytochemical staining of HT-1080 cells with anti-NALP3 antibody (Cat#1274, 1:1,000) . Nuclei were stained blue with DAPI; NALP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NALP3 antibody (Cat#1274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NALP3 antibody (Cat#1274, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NLRP3
货号：

1274
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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