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Anti-CD8 alpha Rabbit Monoclonal Antibody #1280

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Flow cytometric analysis of CD8 alpha expression in HT-1080 cells using CD8 alpha antibody (Cat#1280, 1:2,000). Green, isotype control; red, CD8 alpha.
Immunocytochemical staining of HT-1080 cells with CD8 alpha antibody (Cat#1280, 1:1,000). Nuclei were stained blue with DAPI; CD8 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-CD8 alpha antibody (Cat#1280, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CD8 alpha antibody (Cat#1280). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD8 alpha antibody (Cat#1280, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CD8A
  • 货号：
    1280
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-CD8 alpha Rabbit Monoclonal Antibody
Aliases CD8A; CD8 Subunit Alpha ; T-Cell Surface Glycoprotein CD8 Alpha Chain; CD8alpha; P32; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); CD8a Molecule; CD8; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor; T8 T-Cell Antigen; CD8a Antigen; IMD116; Leu2; MAL
Background

Gene Name: CD8A

NCBI Gene Entry: 925

UniProt Entry: P01732
Application Information

Molecular Weight: Predicted, 26 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1420

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human CD8
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD8 alpha expression in HT-1080 cells using CD8 alpha antibody (Cat#1280, 1:2,000). Green, isotype control; red, CD8 alpha.
  • Immunocytochemical staining of HT-1080 cells with CD8 alpha antibody (Cat#1280, 1:1,000). Nuclei were stained blue with DAPI; CD8 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-CD8 alpha antibody (Cat#1280, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Western blotting analysis using anti-CD8 alpha antibody (Cat#1280). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD8 alpha antibody (Cat#1280, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CD8A
  • 货号：
    1280
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of CD8 alpha expression in HT-1080 cells using CD8 alpha antibody (Cat#1280, 1:2,000). Green, isotype control; red, CD8 alpha.
Immunocytochemical staining of HT-1080 cells with CD8 alpha antibody (Cat#1280, 1:1,000). Nuclei were stained blue with DAPI; CD8 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-CD8 alpha antibody (Cat#1280, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CD8 alpha antibody (Cat#1280). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD8 alpha antibody (Cat#1280, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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