Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody - 产品中心

Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody #1291

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Flow cytometric analysis of PI3 Kinase p85 alpha expression in C2C12 cells using PI3 Kinase p85 alpha antibody (Cat#1291, 1:2,000). Green, isotype control; red, PI3 Kinase p85 alpha.

Immunocytochemical staining of C2C12 cells with anti-PI3 Kinase p85 alpha antibody (Cat#1291, 1:1,000) . Nuclei were stained blue with DAPI; PI3 Kinase p85 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PI3 Kinase p85 alpha antibody (Cat#1291). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p85 alpha antibody (Cat#1291, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PIK3R1
货号：

1291
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
Aliases	PIK3R1; Phosphoinositide-3-Kinase Regulatory Subunit 1; GRB1; P85-ALPHA; P85; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha; Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha); Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Phosphoinositide-3-Kinase Regulatory Subunit Alpha; PtdIns-3-Kinase Regulatory Subunit Alpha; Growth Factor Receptor Bound 1; PI3K Regulatory Subunit Alpha; PI3-Kinase Subunit P85-Alpha; PI3 Kinase-Associated P85; P85alpha; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha); Phosphatidylinositol 3-Kinase-Associated P-85 Alpha; PtdIns-3-Kinase Regulatory Subunit P85-Alpha; PI3-Kinase Regulatory Subunit Alpha; P85ALPHA; IMD36; AGM7
Background	Gene Name: PIK3R1 NCBI Gene Entry: 5295 UniProt Entry: P27986
Application Information	Molecular Weight: Predicted, 84 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2750 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PI3 Kinase p85 alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PI3 Kinase p85 alpha expression in C2C12 cells using PI3 Kinase p85 alpha antibody (Cat#1291, 1:2,000). Green, isotype control; red, PI3 Kinase p85 alpha.
Immunocytochemical staining of C2C12 cells with anti-PI3 Kinase p85 alpha antibody (Cat#1291, 1:1,000) . Nuclei were stained blue with DAPI; PI3 Kinase p85 alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PI3 Kinase p85 alpha antibody (Cat#1291). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PI3 Kinase p85 alpha antibody (Cat#1291, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PIK3R1
货号：

1291
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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