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Anti-Ring finger protein 20 Rabbit Monoclonal Antibody #1306

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Flow cytometric analysis of Ring finger protein 20 expression in HeLa cells using Ring finger protein 20 antibody (Cat#1306, 1:2,000). Green, isotype control; red, Ring finger protein 20.

Immunocytochemical staining of HepG2 cells with Ring finger protein 20 antibody (Cat#1306, 1:1,000). Nuclei were stained blue with DAPI; Ring finger protein 20 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Ring finger protein 20 antibody (Cat#1306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ring finger protein 20 antibody (Cat#1306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RNF20
货号：

1306
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Ring finger protein 20 Rabbit Monoclonal Antibody
Aliases	RNF20; Ring Finger Protein 20; BRE1A; HBRE1; BRE1; RING-Type E3 Ubiquitin Transferase BRE1A; E3 Ubiquitin-Protein Ligase BRE1A; FLJ20382; FLJ11189; KAIA2779; BRE1 E3 Ubiquitin Ligase Homolog (S. Cerevisiae); BRE1 E3 Ubiquitin Ligase Homolog; Homolog Of S. Cerevisiae BRE1; RING Finger Protein 20; EC 2.3.2.27; EC 6.3.2; BRE1-A
Background	Gene Name: RNF20 NCBI Gene Entry: 56254 UniProt Entry: Q5VTR2
Application Information	Molecular Weight: Predicted, 114 kDa; observed, 114 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2555 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RNF20
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Ring finger protein 20 expression in HeLa cells using Ring finger protein 20 antibody (Cat#1306, 1:2,000). Green, isotype control; red, Ring finger protein 20.
Immunocytochemical staining of HepG2 cells with Ring finger protein 20 antibody (Cat#1306, 1:1,000). Nuclei were stained blue with DAPI; Ring finger protein 20 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Ring finger protein 20 antibody (Cat#1306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ring finger protein 20 antibody (Cat#1306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RNF20
货号：

1306
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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