Immunocytochemical staining of HT-1080 cells with anti-BCL2 like 1 antibody(Cat#63370, 1:1,000) . Nuclei were stained blue with DAPI; BCL2 like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-BCL2 like 1 antibody (Cat#63370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL2 like 1 antibody (Cat#63370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-BCL2 like 1 antibody (Cat#63370). BCL2 like 1 expression in wild type (WT) and BCL2 like 1 (BCL2L1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-BCL2 like 1 antibody (Cat#63370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of BCL2 like 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-BCL2 like 1 antibody (Cat#63370, 1:2,000) and analyzed using BD flow cytometer.
