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Anti-CHRNA5 Rabbit Monoclonal Antibody #1438

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Flow cytometric analysis of CHRNA5 expression in HAP-1 cells using CHRNA5 antibody (Cat#1438, 1:2,000). Green, isotype control; red, CHRNA5.

Immunocytochemical staining of HAP-1 cells with CHRNA5 antibody (Cat#1438, 1:1,000). Nuclei were stained blue with DAPI; CHRNA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CHRNA5 antibody (Cat#1438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNA5 antibody (Cat#1438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CHRNA5
货号：

1438
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-CHRNA5 Rabbit Monoclonal Antibody
Aliases	CHRNA5; Cholinergic Receptor Nicotinic Alpha 5 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 5 (Neuronal); Cholinergic Receptor, Nicotinic, Alpha 5 (Neuronal); Neuronal Acetylcholine Receptor Subunit Alpha-5; Cholinergic Receptor, Nicotinic Alpha 5; Cholinergic Receptor, Neuronal Nicotinic, Alpha Polypeptide-5; Neuronal Nicotinic Acetylcholine Receptor, Alpha5 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 5; NACHRA5; LNCR2
Background	Gene Name: CHRNA5 NCBI Gene Entry: 1138 UniProt Entry: P30532
Application Information	Molecular Weight: Predicted, 53 kDa; observed, 55 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3775 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CHRNA5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CHRNA5 expression in HAP-1 cells using CHRNA5 antibody (Cat#1438, 1:2,000). Green, isotype control; red, CHRNA5.
Immunocytochemical staining of HAP-1 cells with CHRNA5 antibody (Cat#1438, 1:1,000). Nuclei were stained blue with DAPI; CHRNA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CHRNA5 antibody (Cat#1438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNA5 antibody (Cat#1438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CHRNA5
货号：

1438
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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