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KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody #67431

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Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#67431, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#67431, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-GSK3 alpha/beta antibody (Cat#67431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 alpha/beta antibody (Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GSK3 a/ antibody (Cat#67431). GSK3 a/ expression in wildtype (WT) and GSK3 a/3 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-GSK3 a/ antibody(Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:20,000)respectively. lmage was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    GSK3A/GSK3B
  • 货号：
    67431
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody
Aliases GSK3A; Glycogen Synthase Kinase 3 Alpha; Serine/Threonine-Protein Kinase GSK3A; Glycogen Synthase Kinase-3 Alpha; EC 2.7.11.26; GSK-3 Alpha; EC 2.7.11.1; EC 2.7.11;Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
Background

Gene Name: GSK3A/GSK3B

NCBI Gene Entry: 2931/2932

UniProt Entry: P49840/P49841
Application Information

Molecular Weight: Predicted, 51 kDa, observed, 51,47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1100

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human GSK3 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#67431, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
  • Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#67431, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-GSK3 alpha/beta antibody (Cat#67431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 alpha/beta antibody (Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-GSK3 a/ antibody (Cat#67431). GSK3 a/ expression in wildtype (WT) and GSK3 a/3 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-GSK3 a/ antibody(Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:20,000)respectively. lmage was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    GSK3A/GSK3B
  • 货号：
    67431
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#67431, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#67431, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-GSK3 alpha/beta antibody (Cat#67431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 alpha/beta antibody (Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GSK3 a/ antibody (Cat#67431). GSK3 a/ expression in wildtype (WT) and GSK3 a/3 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-GSK3 a/ antibody(Cat#67431, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201,1:20,000)respectively. lmage was developed using FeQ™ ECL Substrate Kit (Cat#226).
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