Anti-Alpha Skeletal Muscle Actin Rabbit Monoclonal Antibody - 产品中心

Anti-Alpha Skeletal Muscle Actin Rabbit Monoclonal Antibody #1571

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Flow cytometric analysis of Actin expression in C2C12 cells using Actin antibody (Cat#1571, 1:2,000). Green, isotype control; red, Actin.

Immunocytochemical staining of C2C12 cells with Actin antibody (Cat#1571, 1:1,000). Nuclei were stained blue with DAPI; Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Actin antibody (Cat#1571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Actin antibody (Cat#1571, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ACTA1
货号：

1571
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Alpha Skeletal Muscle Actin Rabbit Monoclonal Antibody
Aliases	Actin Alpha 1, Skeletal Muscle; NEM3; ACTA; Actin, Alpha Skeletal Muscle; Nemaline Myopathy Type 3; Alpha-Actin-1; EC 3.6.4.-; CMYP2A; CMYP2B; CMYP2C; CFTD1; CFTDM; ASMA; CFTD; MPFD; NEM1; NEM2;SHPM
Background	Gene Name: ACTA1 NCBI Gene Entry: 58 UniProt Entry: P68133
Application Information	Molecular Weight: Predicted, 42 kDa; observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1355 Species Reactivity: Mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Actin (Alpha-Actin)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Actin expression in C2C12 cells using Actin antibody (Cat#1571, 1:2,000). Green, isotype control; red, Actin.
Immunocytochemical staining of C2C12 cells with Actin antibody (Cat#1571, 1:1,000). Nuclei were stained blue with DAPI; Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Actin antibody (Cat#1571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Actin antibody (Cat#1571, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ACTA1
货号：

1571
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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