Flow cytometric analysis of Protein tyrosine kinase 2 expression in C2C12 cells using anti-Protein tyrosine kinase 2 antibody (Cat#61560, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2.
lmmunocytochemical staining of C2C12 cells with Protein tyrosine kinase 2 antibody (Cat#61560, 1:1,000). Nuclei were stained blue with DAPl; Protein tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#61560). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#61560, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#61560). Protein tyrosine kinase 2 expression in wild-type (WT) and protein tyrosine kinase 2 (PTK2) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#61560, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
