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Anti-Hsp90 α Rabbit Monoclonal Antibody #1668

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Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Green, isotype control; red, Hsp90 α.
Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HSP90AA1
  • 货号:
    1668
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Hsp90 α Rabbit Monoclonal Antibody
Aliases HSP90AA1; Heat Shock Protein 90 Alpha Family Class A Member 1; HSP90N; HSPC1; HSPCA; Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1; Lipopolysaccharide-Associated Protein; Heat Shock 90kDa Protein 1, Alpha; Renal Carcinoma Antigen NY-REN-38; Heat Shock 90kD Protein 1, Alpha; Heat Shock Protein HSP 90-Alpha; LPS-Associated Protein; Heat Shock 86 KDa; FLJ31884; HSP90A; HSP 86; Hsp89; Hsp90; HSP86; LAP-2; Heat Shock Protein 90kDa Alpha Family Class A Member 1; Epididymis Secretory Sperm Binding Protein Li 65p; Epididymis Luminal Secretory Protein 52; Heat Shock 90kD Protein 1, Alpha-Like 4; Heat Shock 90kD Protein, Alpha-Like 4; EC 3.6.4.10; HEL-S-65p; HSPCAL1; HSPCAL4; HSP89A; Hsp103; HSP89; HSP90; EL52; HSPN; LAP2
Background

Gene Name: HSP90AA1

NCBI Gene Entry: 3320

UniProt Entry: P07900

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3995

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Hsp90 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Green, isotype control; red, Hsp90 α.
  • Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Green, isotype control; red, Hsp90 α.
Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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