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Anti-ATP2A1 Rabbit Monoclonal Antibody #1758

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Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.
Western blotting analysis using anti-ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ATP2A1
  • 货号：
    1758
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-ATP2A1 Rabbit Monoclonal Antibody
Aliases ATP2A1; ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1; SERCA1; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1; Calcium Pump 1; ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SR Ca(2+)-ATPase 1; ATP2A; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isoform; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3
Background

Gene Name: ATP2A1

NCBI Gene Entry: 487

UniProt Entry: O14983
Application Information

Molecular Weight: Predicted, 110 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4340

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ATP2A1/SERCA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.
  • Western blotting analysis using anti-ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ATP2A1
  • 货号：
    1758
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HepG2 cells with ATP2A1 antibody (Cat#1758, 1:1,000). Nuclei were stained blue with DAPI; ATP21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.
Western blotting analysis using anti-ATP2A1 antibody (Cat#1758). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2A1 antibody (Cat#1758, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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