Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).