Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody (Cat#71204, 1:2,000). Green, isotype control; red, Desmocollin 3.
Immunocytochemical staining of Hela cells with anti-Desmocollin 3 antibody (Cat#71204, 1:1,000). Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-desmocollin 3 antibody (Cat#71204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-desmocollin 3 antibody (Cat#71204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-desmocollin 3 antibody (Cat#71204). Desmocollin 3 expression in wild-type (WT) and desmocollin 3 (DSC3) knockout (KO) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-desmocollin 3 antibody (Cat#71204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).