Anti-Secreted frizzled related protein 4 Rabbit Monoclonal Antibody - 产品中心

Anti-Secreted frizzled related protein 4 Rabbit Monoclonal Antibody #1868

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Flow cytometric analysis of Secreted frizzled related protein 4 expression in HepG2 cells using Secreted frizzled related protein 4 antibody (Cat#1868, 1:2,000). Green, isotype control; red, Secreted frizzled related protein 4.

Immunocytochemical staining of HepG2 cells with anti-Secreted frizzled related protein 4 antibody (Cat #1868, 1:1,000) . Nuclei were stained blue with DAPI; Secreted frizzled related protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-Secreted frizzled related protein 4 antibody (Cat#1868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Secreted frizzled related protein 4 antibody (Cat#1868, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SFRP4
货号：

1868
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Secreted frizzled related protein 4 Rabbit Monoclonal Antibody
Aliases	SFRP4; Secreted Frizzled Related Protein 4; FRPHE; Secreted Frizzled-Related Protein 4; FRZB-2; FRP-4; Frizzled Protein, Human Endometrium; SFRP-4; FrpHE; Secreted Frizzled-Related Protein 4; Secreted Frizzled-Related Protein 4; PYL
Background	Gene Name: SFRP4 NCBI Gene Entry: 6424 UniProt Entry: Q6FHJ7
Application Information	Molecular Weight: Predicted, 40 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4950 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human SFRP4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Secreted frizzled related protein 4 expression in HepG2 cells using Secreted frizzled related protein 4 antibody (Cat#1868, 1:2,000). Green, isotype control; red, Secreted frizzled related protein 4.
Immunocytochemical staining of HepG2 cells with anti-Secreted frizzled related protein 4 antibody (Cat #1868, 1:1,000) . Nuclei were stained blue with DAPI; Secreted frizzled related protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Secreted frizzled related protein 4 antibody (Cat#1868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Secreted frizzled related protein 4 antibody (Cat#1868, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SFRP4
货号：

1868
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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