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Anti-ASH2L Rabbit Monoclonal Antibody #1732

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Flow cytometric analysis of ASH2L expression in C2C12 cells using ASH2L antibody (Cat#1732, 1:2,000). Green, isotype control; red, ASH2L.
Immunocytochemical staining of C2C12 cells with ASH2L antibody (Cat#1732, 1:1,000). Nuclei were stained blue with DAPI; ASH2L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ASH2L antibody (Cat#1732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASH2L antibody (Cat#1732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ASH2L,ASH2 like, histone lysine methyltransferase complex subunit.
  • 基因名:
    ASH2L
  • 货号:
    1732
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ASH2L Rabbit Monoclonal Antibody
Aliases ASH2L; ASH2 Like, Histone Lysine Methyltransferase Complex Subunit; ASH2L2; ASH2L1; ASH2; Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2; ASH2-Like Protein; Bre2; ASH2 Like Histone Lysine Methyltransferase Complex Subunit; Ash2 (Antibodysent, Small, Or Homeotic)-Like (Drosophila); Ash2 (Antibodysent, Small, Or Homeotic)-Like; BRE2
Background

Gene Name: ASH2L

NCBI Gene Entry: 9070

UniProt Entry: Q9UBL3

Application Information

Molecular Weight: Predicted, 69 kDa; observed, 69 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1485

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ASH2L
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ASH2L expression in C2C12 cells using ASH2L antibody (Cat#1732, 1:2,000). Green, isotype control; red, ASH2L.
  • Immunocytochemical staining of C2C12 cells with ASH2L antibody (Cat#1732, 1:1,000). Nuclei were stained blue with DAPI; ASH2L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ASH2L antibody (Cat#1732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASH2L antibody (Cat#1732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ASH2L,ASH2 like, histone lysine methyltransferase complex subunit.
Citation(0)
Flow cytometric analysis of ASH2L expression in C2C12 cells using ASH2L antibody (Cat#1732, 1:2,000). Green, isotype control; red, ASH2L.
Immunocytochemical staining of C2C12 cells with ASH2L antibody (Cat#1732, 1:1,000). Nuclei were stained blue with DAPI; ASH2L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ASH2L antibody (Cat#1732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASH2L antibody (Cat#1732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ASH2L,ASH2 like, histone lysine methyltransferase complex subunit.
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